DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

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DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)

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dc.contributor.author Hastings, J. M.
dc.contributor.author Schultheis, P. J.
dc.contributor.author Whitson, M.
dc.contributor.author Holliday, Charles W.
dc.contributor.author Coelho, J. R.
dc.contributor.author Mendell, A. M.
dc.date.accessioned 2010-02-17T16:28:29Z
dc.date.available 2010-02-17T16:28:29Z
dc.date.issued 2008
dc.identifier.citation Hastings, J. M., Schultheis, P. J., et.al. (2008.) "DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae)." Zootaxa 1713: 27-38. en_US
dc.identifier.uri http://hdl.handle.net/10385/598
dc.description.abstract We are engaged in a comprehensive study of cicada killers (Sphecius spp.), including their behavioral ecology. At one location, we observed interactions among three putative species of Sphecius, and used DNA barcoding to help clarify relationships among them. For this, we sequenced a fragment of the mitochondrial cytochrome c oxidase subunit I gene. During our study, a new taxonomic key of New World cicada killers, based on morphology, was published, and we expanded the barcoding project to test the congruence between barcodes and this key. In general, barcoding evidence supports morphological distinctions among species; sequence divergences between individuals of different species were within the range expected for congeneric Hymenoptera. However, two conflicts between barcoding and morphological evidence were noted. 1) Haplotypes of Sphecius grandis Say fall into two highly divergent clades, suggesting they are cryptic species. 2) Two clades of S. convallis Patton were found, and the sequences of one clade are virtually identical to those of S. speciosus Drury, suggesting that this clade of S. convallis is conspecific with S. speciosus. Alternative explanations are possible for this result, including hybridization and introgression between the two species. We conclude that our DNA barcoding evidence should be interpreted with caution, but that it has generated interesting questions we hope to resolve with field research coupled with analysis of suitable nuclear gene sequences. en_US
dc.publisher Zootaxa en_US
dc.subject cytochrome c oxidase subunit I en_US
dc.subject sympatric species en_US
dc.subject Big Bend National Park en_US
dc.subject introgression en_US
dc.subject cryptic species en_US
dc.title DNA barcoding of new world cicada killers (Hymenoptera: Crabronidae) en_US
dc.type Article en_US

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