Murine mammary carcinoma cells and CD11c+ dendritic cells elicit distinct responses to lipopolysaccharide and exhibit differential expression of genes required for TLR4 signaling

Although TLR are often studied on DC because of their ability to bridge innate and adaptive defenses, TLR are also expressed by epithelial cells. Because the majority of cancers are carcinomas, and thus of epithelial origin, we wanted to know whether a carcinoma and DC responded similarly to a TLR agonist. We found the mammary carcinoma 4T1 and CD11c+ DC both secreted proinflammatory chemokines in response to the TLR4 agonist lipopolysaccharide (LPS). However a clear dichotomy existed. DC, but not 4T1 secreted IL-1β, TNF-α, and upregulated CD80 and CD86 expression following LPS treatment. A potential reason for differential responsiveness was that DC expressed greater levels of TLR4, CD14, Myd88, and TRAM. Despite the low level of TLR signaling proteins, the carcinoma were able to elicit a range of responses contingent upon the source, dose, length, and frequency of TLR agonist treatment. Thus, carcinoma and DC are distinctly responsive to LPS.

Title	Murine mammary carcinoma cells and CD11c+ dendritic cells elicit distinct responses to lipopolysaccharide and exhibit differential expression of genes required for TLR4 signaling
Creator	Sgroe, E. P.
	Blum, C. M.
	Palha De Sousa, C.
	Crespo, A. M.
	Kurt, Robert A.
Publisher	Cellular Immunology
Academic Department	Biology
Division	Natural Sciences
Organization	Lafayette College
Date Issued	2010
Date Available	2015-06-05T18:57:03Z
Type	Article
Language	English
Keyword	dendritic cells
	LPS
	toll-like receptors
	tumor cells
Bibliographic Citation	Palha De Sousa, C., et al. (2010) "Murine mammary carcinoma cells and CD11c+ dendritic cells elicit distinct responses to lipopolysaccharide and exhibit differential expression of genes required for TLR4 signaling." Cellular Immunology 266 (1): 67-75.
Standard Identifier	Handle 10385/1990
Standard Identifier	DOI 10.1016/j.cellimm.2010.08.015
Permalink	http://hdl.handle.net/10385/1990
Rights Statement	In Copyright
Rights Holders	Elsevier Inc.