Postmetacercarial changes in Echinostoma caproni maintained in a defined medium plus calf serum
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Abstract
- The present study examined postmetacercarial changes in the excysted metacercariae of Echinostoma caproni maintained in the defined medium Mixture 199 plus 20% calf serum for 7 days at 41℃. The gas phase was atmospheric air. Each culture was inoculated with 25 excysted metacerariae. Cultures were maintained upright in closed 15 ml plastic centrifuge tubes each containing 10 ml of medium plus 200 units of penicillin/ml and 200 µg of streptomycin/ml. By 4 days in culture, most metacercariae had voided their excretory concretions. Organisms were clumped or solitary at the bottom of the cultures. Many organisms showed flaring of the oral collar and extension of both the collar and tegumentary spines. By 4 days in culture, posterior protuberances or bumps were noted on many of the organisms and some organisms showed abnormal vesicular growths or blebs at their posterior ends. Some mortality was noted in culture by day 5, but most organisms were still alive when the cultures were terminated on day 7.
| Title | Postmetacercarial changes in Echinostoma caproni maintained in a defined medium plus calf serum |
|---|---|
| Creator | Fried, Bernard |
| Reddy, A. | |
| Publisher | Korean Journal of Parasitology |
| Academic Department | Biology |
| Division | Natural Sciences |
| Organization | Lafayette College |
| Date Issued | September 2000 |
| Date Available | 2015-06-05T15:05:56Z |
| Type | Article |
| Language | English |
| Keyword | excysted metacercariae |
| defined medium plus serum | |
| abnormal body growths | |
| Echinostoma caproni | |
| in vitro culture | |
| excretory concretions | |
| Trematoda | |
| Bibliographic Citation | Fried, B. and A. Reddy (2000 Sept.) "Postmetacercarial changes in Echinostoma caproni maintained in a defined medium plus calf serum." Korean Journal of Parasitology 38 (3): 173–175. |
| Standard Identifier | Handle 10385/1981 |
| DOI 10.3347/kjp.2000.38.3.173 | |
| Permalink | http://hdl.handle.net/10385/1981 |
| Rights Statement |
Creative Commons - Attribution, Non-Commercial
|
| Rights Holders | The Korean Society for Parasitology and Tropical Medicine |
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| File | Fried-KoreanJournalofParasitology-vol38-2000.pdf | Uploaded 2019-10-20 | Public | Download |
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