Sensitivity evaluation of two human breast cancer cell lines to tamoxifen through apoptosis induction

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Abstract
  • Tamoxifen citrate (TAM) has been used to treat breast cancer in women for many years. The comparative
    effects of TAM in inducing apoptosis were evaluated in estrogen receptor-positive (ER-positive
    MCF-7) and estrogen receptor-negative (ER-negative MDA-MB-231) human breast cancer
    cell lines in vitro in order to determine if these two cell lines differ in their sensitivity to TAM. Mitochondrial
    membrane permeability potential disruption was assessed in both cell lines by a lipophilic
    cationic dye (DePsipher assay, Trevigen, Inc.) utilizing fluorescence microscopy. Using
    this specific fluorochrome, we were able to associate mitochondrial membrane disruption to early,
    mid-, and late apoptotic cells. TAM induced cell death via apoptosis in both ER-positive and ER-negative
    cells, however, apoptosis induction was more pronounced in ER-positive MCF-7 compared
    to ER-negative MDA-MB-231 breast cancer cells. These findings may have some therapeutic
    use in the treatment of estrogen dependent and estrogen independent breast cancer.
TitleSensitivity evaluation of two human breast cancer cell lines to tamoxifen through apoptosis induction
CreatorAzuelos, C.
Majumdar, Shyamal K.
Keene, S.
PublisherOpen Journal of Apoptosis
Academic DepartmentBiology
DivisionNatural Sciences
OrganizationLafayette College
Date Issued2014
Date Available2015-05-19T20:30:38Z
TypeArticle
LanguageEnglish
KeywordMCF-7 and MDA-MB-231 human breast cancer cell lines
mitochondrial membrane potential assay
estrogen receptor
Tamoxifen
apoptosis
Bibliographic CitationKeene, S., C. Azuelos, and S. K. Majumdar (2014) "Sensitivity evaluation of two human breast cancer cell lines to tamoxifen through apoptosis induction." Open Journal of Apoptosis 3 (4): 50850.
Standard IdentifierHandle 10385/1939
DOI http://dx.doi.org/10.4236/ojapo.2014.34008
Permalinkhttp://hdl.handle.net/10385/1939
Rights Statement Creative Commons - Attribution Creative Commons - Attribution
Rights HoldersScientific Research Publishing Inc.
Azuelos, C.
Majumdar, Shyamal K.
Keene, S.
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