Effects of different levels of Echinostoma caproni miracidial dose on glucose and maltose composition of Biomphalaria glabrata snails as determined by high performance thin-layer chromatography-densitometry
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Abstract
- The effects of 5, 25, and 40 Echinostoma caproni miracidia on the sugar content
of young adult and mature adult Biomphalaria glabrata were studied using high
performance thin layer chromatography (HPTLC)-densitometry. Analysis was done on the
snail’s digestive gland gonad complex (DGG) at two and four weeks postmiracidial
exposure. The sugars were extracted from the DGG using 70% ethanol and analyzed on
silica gel HPTLC plates with a preadsorbent zone using 1-butanol-glacial acetic acid-diethyl
ether-deionized water (27:18:5:3) mobile phase. The separated bands were then detected
using alpha-naphthol-sulfuric reagent and quantified by densitometry at 515 nm. Significant
differences were found in the maltose content between two and four weeks post exposure
for both age groups. Additionally, significantly lower maltose and glucose levels were
observed in the high exposure groups of both ages.
| Title | Effects of different levels of Echinostoma caproni miracidial dose on glucose and maltose composition of Biomphalaria glabrata snails as determined by high performance thin-layer chromatography-densitometry |
|---|---|
| Creator | Fried, Bernard |
| DeGrandchamp, D. | |
| Sherma, Joseph | |
| Hartlaub, S. | |
| Publisher | Chromatography |
| Academic Department | Biology |
| Chemistry | |
| Division | Natural Sciences |
| Organization | Lafayette College |
| Date Issued | 2015 |
| Date Available | 2015-05-19T15:39:55Z |
| Type | Article |
| Language | English |
| Keyword | digestive-gland gonad complex |
| thin-layer chromatography | |
| Echinostoma caproni | |
| sugars | |
| miracidia | |
| Biomphalaria glabrata | |
| glucose | |
| DGG | |
| maltose | |
| Bibliographic Citation | DeGrandchamp, D., et al. (2015) "Effects of different levels of Echinostoma caproni miracidial dose on glucose and maltose composition of Biomphalaria glabrata snails as determined by high performance thin-layer chromatography-densitometry." Chromatography 2 (2): 188-94. |
| Standard Identifier | Handle 10385/1937 |
| Permalink | http://hdl.handle.net/10385/1937 |
| Rights Statement |
Creative Commons - Attribution
|
| Rights Holders | Fried, Bernard |
| DeGrandchamp, D. | |
| Sherma, Joseph | |
| Hartlaub, S. |
Contains
| File | Fried-Chromatography-vol2-no2-2015.pdf | Uploaded 2019-10-20 | Public | Download |
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