High-performance thin-layer chromatographic analysis of the neutral lipid content of urine and feces in mice experimentally infected with Schistosoma mansoni

The purpose of this study was to identify and quantify neutral lipids in the urine and feces of mice experimentally infected with Schistosoma mansoni. Four infected and three control mice were used in this study. The samples were collected weekly for eight weeks. Samples from the infected mice were compared to those from the uninfected ones. The analyzed lipids in both feces and urine samples were free sterols, free fatty acids, triacylglycerols, and methyl esters. Steryl esters were also analyzed in fecal samples. The neutral lipids were separated on channeled preadsorbant Analtech HPTLC-HLF 20 x 10-cm silica gel plates with petroleum ether-diethyl ether-glacial acetic acid (80:20:1) mobile phase and detected with 5% ethanolic phosphomolybdic acid spray reagent. Quantification was carried out by use of a CAMAG Scanner 3 in the visible mode. Significant differences between infected and uninfected samples were found in methyl esters of feces after four weeks, and steryl esters of feces after five weeks. Also, triacylglycerols in urine samples were statistically different at eight weeks in infected versus uninfected samples. The results of our study suggest that certain neutral lipids in the urine and feces of mice may serve as indicators of infection with S. mansoni.

Title	High-performance thin-layer chromatographic analysis of the neutral lipid content of urine and feces in mice experimentally infected with Schistosoma mansoni
Creator	Fried, Bernard
	Bolstridge, J.
	Cicchi, M.
	Popovic, N.
	Sherma, Joseph
Publisher	Trends in Chromatography
Academic Department	Biology
Academic Department	Chemistry
Division	Natural Sciences
Organization	Lafayette College
Date Issued	2013
Date Available	2015-02-03T18:46:43Z
Type	Article
Language	English
Keyword	thin-layer chromatography
	neutral lipids
	mice
	Schistosoma mansoni
Bibliographic Citation	Cicchi, M., et al. (2013) "High-performance thin-layer chromatographic analysis of the neutral lipid content of urine and feces in mice experimentally infected with Schistosoma mansoni." Trends in Chromatography 8: 1-6.
Standard Identifier	Handle 10385/1581
Permalink	http://hdl.handle.net/10385/1581
Rights Statement	In Copyright