Comparative study of the quantification of thin-layer chromatograms of a model dye using three types of commercial densitometers and image analysis with ImageJ

A comparison of slit scanning densitometry, videodensitometry, flatbed scanner densitometry, and image analysis is presented for quantification of high performance thin layer chromatograms containing zones of the model dye rhodamine B. For the first three methods commercial instruments were used, i.e, CAMAG TLC Scanner 3, CAMAG Videodensitometer, and AR2i ChromImage flatbed scanner, respectively. Image analysis was performed using a digital camera and free ImageJ software. Information is provided on the construction and applications of the commercial instruments, and a review of recent publications on thin layer chromatography with image analysis is provided.

Title	Comparative study of the quantification of thin-layer chromatograms of a model dye using three types of commercial densitometers and image analysis with ImageJ
Creator	Sherma, Joseph
Creator	Popovic, N.
Publisher	Trends in Chromatography
Academic Department	Chemistry
Division	Natural Sciences
Organization	Lafayette College
Date Issued	2014
Date Available	2015-02-03T18:28:48Z
Type	Article
Language	English
Keyword	ImageJ
	TLC
	thin layer chromatography
	image analysis
	ChromImage
	videodensitometry
	densitometry
	planar chromatography
Bibliographic Citation	Popovic, N. and J. Sherma (2014) "Comparative study of the quantification of thin-layer chromatograms of a model dye using three types of commercial densitometers and image analysis with ImageJ." Trends in Chromatography 9: 21-28.
Standard Identifier	Handle 10385/1580
Permalink	http://hdl.handle.net/10385/1580
Rights Statement	In Copyright