Isolation and characterization of a mutant of Chlamydomonas reinhardtii deficient in the CO2 concentrating mechanism

A Chlamydomonas reinhardtii mutant has been isolated that cannot grow photoautotrophically on low CO2 concentrations but can grow on elevated CO2. In a test cross, the high CO2-requirement for growth showed a 2:2 segregation. This mutant, designated CIA-5, had a phenotype similar to previously identified mutants that were defective in some aspect of CO2 accumulation. Unlike previously isolated mutants, CIA-5 did not have detectable levels of the periplasmic carbonic anhydrase, an inducible protein that participates in the acquisition of CO2 by C. reinhardtii. CIA-5 also did not accumulate inorganic carbon to levels higher than could be accounted for by diffusion. This mutant strain did not synthesize any of the four polypeptides preferentially made by wild type C. reinhardtii when switched from an environment containing elevated CO2 levels to an environment low in CO2. It is concluded that this mutant fails to induce the CO2 concentrating system and is incapable of adapting to low CO2 conditions.

Title	Isolation and characterization of a mutant of Chlamydomonas reinhardtii deficient in the CO2 concentrating mechanism
Creator	Manuel, L. J.
	Kitayama, M.
	Togosaki, R. K.
	Husic, H. David
	Tolbert, N. E.
	Moroney, J. V.
Publisher	Plant Physiology
Academic Department	Chemistry
Division	Natural Sciences
Organization	Lafayette College
Date Issued	1989
Date Available	2013-01-29T20:31:17Z
Type	Article
Language	English
Bibliographic Citation	Moroney, J. V., et al. (1989) "Isolation and characterization of a mutant of Chlamydomonas reinhardtii deficient in the CO2 concentrating mechanism." Plant Physiology 89 (3): 897-903.
Standard Identifier	DOI 10.1104/pp.89.3.897
Standard Identifier	Handle 10385/1074
Permalink	http://hdl.handle.net/10385/1074
Rights Statement	In Copyright
Rights Holders	American Society of Plant Physiologists