Encystment and excystment of the paramphistomid trematode Zygocotyle lunata

This study reports further observations on encystment and excystment of the paramphistomid trematode Zygocotyle lunata. Of numerous substrates tested in the laboratory for cercarial encystment, i.e. plastic and glass dishes, Styrofoam (TM), aluminium foil, snail shells, and cheesecloth, all but cheesecloth allowed for 100% encystment within 4 h of cercarial release at 28 degrees C. Numerous physical and chemical factors were tested to kill cysts, and their capacity to do so was determined by the subsequent inability of cysts to excyst within a chemical excystment medium. Vinegar, Italian salad dressing, soy sauce and 10% solutions of sucrose, acetic acid and sodium chloride were the most detrimental. Freezing, boiling and storage of cysts for more than 2 years also killed them. Several marinades such as lemon juice, Worcestershire sauce, and various concentrations of potassium permanganate, did not kill the majority of cysts tested. Since Z. lunata is a potential foodborne trematode for humans and animals, it is important to determine suitable ways to control or kill these cysts.

Title	Encystment and excystment of the paramphistomid trematode Zygocotyle lunata
Creator	Fried, Bernard
Creator	LeSage, K. E.
Publisher	Journal of Helminthology
Academic Department	Biology
Division	Natural Sciences
Organization	Lafayette College
Date Issued	September 2011
Date Available	2013-01-25T21:54:19Z
Type	Article
Language	English
Bibliographic Citation	LeSage, K. E. and B. Fried. (2011 September). "Encystment and excystment of the paramphistomid trematode Zygocotyle lunata." Journal of Helminthology 85 (3): 300-303.
Standard Identifier	Handle 10385/1064
Standard Identifier	DOI 10.1017/S0022149X10000593
Permalink	http://hdl.handle.net/10385/1064
Rights Statement	In Copyright
Rights Holders	Cambridge University Press